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Protein Expression Associate

Job ID: req1070
Facility: Frederick: ATRF
Location: 8560 Progress Dr, Frederick, MD 21701 USA

The Frederick National Laboratory is dedicated to improving human health through discovery and innovation in the biomedical sciences, focusing on cancer, AIDS, and emerging infectious diseases. 

Position Overview:

PROGRAM DESCRIPTION
The Cancer Research Technology Program (CRTP) develops and implements emerging technology, cancer biology expertise and research capabilities to accomplish NCI research objectives. The CRTP is an outward-facing, multi-disciplinary hub purposed to enable the external cancer research community. A major focus of the CRTP is the NCI RAS Initiative with the goal to discover new therapeutic interventions against RAS-driven cancers. In addition, the CRTP hosts the Nanotechnology Characterization Laboratory (NCL) which performs and standardizes preclinical efficacy and toxicity testing of nanoparticles intended for cancer therapeutics and diagnostics, and the Antibody Characterization Lab (ACL) which characterizes monoclonal antibodies or other renewable affinity binding reagents for use in cancer related research. CRTP scientists also work collaboratively with intramural NCI investigators to provide research technologies and expertise.

KEY ROLES/RESPONSIBILITIES

The Protein Expression Laboratory (PEL) provides support to the NCI RAS Initiative in the generation of high quality protein reagents for biochemical, biophysical, structural, and drug discovery research. The PEL creates expression constructs, expresses the encoded recombinant proteins in multiple expression systems, and purifies the recombinant proteins for use in a wide variety of applications from X-ray crystallography to drug screening to biochemical analysis. The Protein Expression Associate will:

  • Carry out experiments, under the review of a scientist, in the areas of prokaryotic and eukaryotic protein expression
  • Implement molecular biology processes for the generation of purified proteins
  • Carry out bacterial fermentation using bioreactors
  • Perform microscale protein purification scouting and cell lysis procedures
  • Troubleshoot current processes within the laboratory with an eye towards process improvement
  • Provide timely updates of project progress to supervisor and other staff in both informal and formal reports
  • Maintain detailed records of all laboratory processes and procedures for quality assurance purposes

BASIC QUALIFICATIONS

  • Possession of a Bachelor’s degree from an accredited college or university according to the Council for Higher Education Accreditation.  Foreign degrees must be evaluated for U.S. equivalency
  • In addition to the education requirement, a minimum of 2 years of relevant experience in molecular biology, biochemistry, or related disciplines
  • Previous experience in prokaryotic protein expression
  • Must be able to obtain and maintain a security clearance

PREFERRED QUALIFICATIONS 

  • Prior experience in bacterial expression using baffled shake flasks
  • Experience in bacterial fermentation using bioreactors
  • Ability to handle multiple scientific projects with a high degree of accuracy

JOB HAZARDS

  •  This position is subject to working with or have potential for exposure to infectious material, requiring medical clearance and immunizations

Equal Opportunity Employer (EOE) | Minority/Female/Disabled/Veteran (M/F/D/V) | Drug Free Workplace (DFW)