Post Doctoral Fellow, Small RNA
Job ID: req1006
Facility: Frederick: Ft Detrick
Location: PO Box B, Frederick, MD 21702 USA
The Frederick National Laboratory is dedicated to improving human health through discovery and innovation in the biomedical sciences, focusing on cancer, AIDS, and emerging infectious diseases.
The Laboratory of Human Retrovirology and Immunoinformatics (LHRI), Clinical Services Program, Applied/ Developmental Directorate, has investigated mechanism of innate immune response against HIV, HSV, HCV or other virus infection using techniques of molecular biology, virology, cellular biology and bioinformatics under collaboration with National Institute of Allergy and Infectious Diseases (NIAID)/NIH. A goal of the study is to elucidate virus pathogenesis and host response to support on-going clinical studies in NIAID for HIV or other virus-infected patients. During a course of studies, LHRI has found that Ku70 (a DNA repair protein) is a cytosolic DNA sensor protein which specifically induces interferon-lambda1 from human cells in response to DNA virus infection via a novel signaling pathway and found that it can suppress HIV in macrophages and dendritic cells. Focusing on modulation gene activation, we have demonstrated that small none-targeting RNA (snRNA/siRNA) enhances DNA-mediated induction through a crosstalk of RIG-1 and IFI16. And more recently, we discovered an snRNA with a unique motif sequence potently regulates DNA-induced innate immune response.
The Post-Doctoral Fellow:
- Will characterize the impact of co-infection of DNA virus on HIV replication/pathogenesis
- Will investigate the mechanism of immune escape by DNA or RNA virus in human primary cells
- Present findings in scientific conference(s) and to make multiple publications
Fellowship appointments are made for one year, renewable annually, with an anticipated
period of up to three years.
To be considered for this position, you must minimally meet the knowledge, skills, and abilities listed below:
- Possession of a PhD or MD/PhD degree from an accredited college or university according to the Council for Higher Education Accreditation. Foreign degrees must be evaluated for U.S. equivalency
- Experience in molecular biology and immunology or virology (e.g. cloning of gene, constructing expression plasmids, point mutagenesis, WB, q(RT-)PCR, ELISA)
- Must be able to obtain and maintain a security clearance
Candidates with these desired skills will be given preferential consideration:
- Co-immunoprecipitation (co-IP), over expression of recombinant protein in mammalian cells, qRT-PCR for detecting microRNA
- Production of lenti virus
- Luciferase assay, confocal microscopic analysis
- PFU assay, FRET/Alpha screening assay
- Handling of HIV, HIV-2, HTLV-1, HSV-1, HSV-2, KHSV, CMV, other virus
- This position is subject to working with or have potential for exposure to infectious material, requiring medical clearance and immunizations
Equal Opportunity Employer (EOE) | Minority/Female/Disabled/Veteran (M/F/D/V) | Drug Free Workplace (DFW)